INVESTIGADORES
GARCIA Guillermo Manuel
artículos
Título:
Pyrosequencing to Detect Mutations in FKS1 that Confer Reduced Echinocandin
Autor/es:
WIEDERHOLD, NATHAN P.; GRABINSKI, JODI L.; GARCIA-EFFRON, GUILLERMO; PERLIN, DAVID S.; LEE, SAMUEL A.
Revista:
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Editorial:
American Society for Microbiology
Referencias:
Lugar: Washington DC (EEUU); Año: 2008 vol. 52 p. 4145 - 4148
ISSN:
0066-4804
Resumen:
Pyrosequencing was compared to Sanger dideoxy sequencing to detect mutations in FKS1 responsible for reduced echinocandin susceptibility in Candida albicans. These methods were in complete agreement for 10 of 12 clinical isolates with elevated echinocandin MICs, supporting the potential feasibility of pyrosequencing to detect mutations within diploid fungi.
