INVESTIGADORES
GARCIA Guillermo Manuel
artículos
Título:
Targeted gene disruption of the 14-alpha sterol demethylase (cyp51A) in Aspergillus fumigatus and its role in azole drugs susceptibility
Autor/es:
MELLADO, EMILIA; GARCÍA-EFFRON, GUILLERMO; BUITRAGO, MARÍA JOSÉ; ALCÁZAR-FUOLI, LAURA; CUENCA-ESTRELLA, MANUEL; RODRÍGUEZ-TUDELA, JUAN LUIS
Revista:
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Editorial:
American Society for Microbiology
Referencias:
Lugar: Washington DC (EEUU); Año: 2005 vol. 49 p. 2536 - 2538
ISSN:
0066-4804
Resumen:
The role of Aspergillus fumigatus 14alpha-sterol demethylase (Cyp51A) in azole drug susceptibility was assessed. Targeted disruption of cyp51A in azole-susceptible and -resistant strains decreased MICs from 2- to 40-fold. The cyp51A mutants were morphologically indistinguishable from the wild-type strain, retaining the ability to cause pulmonary disease in neutropenic mice.
