INVESTIGADORES
ARENA Miriam Elizabeth
artículos
Título:
Histological events during in vitro rooting of Nothofagus nervosa (Fagaceae)
Autor/es:
G MARTINEZ PASTUR; M ARENA; L HERNANDEZ; N CURVETTO; E ELIASCO
Revista:
NEW ZEALAND JOURNAL OF BOTANY
Editorial:
THE ROYAL SOCIETY OF NEW ZEALAND
Referencias:
Lugar: Wellington, Nueva Zelanda; Año: 2005 vol. 43 p. 61 - 70
ISSN:
0028-825X
Resumen:
Abstract In vitro rooting protocols were previously
performed on Nothofagus nervosa to optimise
the culture medium and environmental conditions.
A qualitative and quantitative tissue differentiation
study during adventitious rooting in N. nervosa under
different light/dark conditions was carried out to
increase knowledge of the rhizogenesis process in
shoots. The histological events through the rooting
period were consistent with the proposed biochemical
markers (peroxidases, tissue nutrient contents,
polyamines, flavonoids). Development of parenchymatic
tissues, shown by the staining pattern of the
cell contents, and cambium activity were the best
tissues to define the rooting phases. Knowledge of
the histological changes could be used to optimise
a protocol for micro-propagation improving timing,
quantity, and quality of roots, which would positively
affect the survival rate during hardening.In vitro rooting protocols were previously
performed on Nothofagus nervosa to optimise
the culture medium and environmental conditions.
A qualitative and quantitative tissue differentiation
study during adventitious rooting in N. nervosa under
different light/dark conditions was carried out to
increase knowledge of the rhizogenesis process in
shoots. The histological events through the rooting
period were consistent with the proposed biochemical
markers (peroxidases, tissue nutrient contents,
polyamines, flavonoids). Development of parenchymatic
tissues, shown by the staining pattern of the
cell contents, and cambium activity were the best
tissues to define the rooting phases. Knowledge of
the histological changes could be used to optimise
a protocol for micro-propagation improving timing,
quantity, and quality of roots, which would positively
affect the survival rate during hardening.Nothofagus nervosa to optimise
the culture medium and environmental conditions.
A qualitative and quantitative tissue differentiation
study during adventitious rooting in N. nervosa under
different light/dark conditions was carried out to
increase knowledge of the rhizogenesis process in
shoots. The histological events through the rooting
period were consistent with the proposed biochemical
markers (peroxidases, tissue nutrient contents,
polyamines, flavonoids). Development of parenchymatic
tissues, shown by the staining pattern of the
cell contents, and cambium activity were the best
tissues to define the rooting phases. Knowledge of
the histological changes could be used to optimise
a protocol for micro-propagation improving timing,
quantity, and quality of roots, which would positively
affect the survival rate during hardening.N. nervosa under
different light/dark conditions was carried out to
increase knowledge of the rhizogenesis process in
shoots. The histological events through the rooting
period were consistent with the proposed biochemical
markers (peroxidases, tissue nutrient contents,
polyamines, flavonoids). Development of parenchymatic
tissues, shown by the staining pattern of the
cell contents, and cambium activity were the best
tissues to define the rooting phases. Knowledge of
the histological changes could be used to optimise
a protocol for micro-propagation improving timing,
quantity, and quality of roots, which would positively
affect the survival rate during hardening.