INVESTIGADORES
CALVO Ernesto Julio
artículos
Título:
WIRED-ENZYME CORE-SHELL Au NANOPARTICLE BIOSENSOR
Autor/es:
PABLO SCODELLER; VICTORIA FLEXER; RAFAEL SZAMOCKI; ERNESTO J. CALVO; NICOLAS TOGNALLI; ALEX FAINSTEIN; HORACIO TROIANI
Revista:
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Editorial:
American Chemical Society
Referencias:
Lugar: Pensylvania; Año: 2008 vol. 130 p. 12690 - 12697
ISSN:
0002-7863
Resumen:
We report a fully integrated core-shell nanoparticle system responsive to glucose. The system is
comprised of self-assembled glucose oxidase and an osmium molecular wire on core-shell Au nanoparticles.
Characterization of the functional nanoparticles by spectroscopy, quartz crystal microbalance
and electrochemical techniques has shown that the catalytically active shell has a structure as designed
and all components are active in the self-assembled multilayer shell. Furthermore, amperometric
reagentless detection of glucose and contactless photonic biosensing by the Os(II) resonant Raman
signal have been demonstrated. The enzymatic reduction of FAD by glucose and further reduction of the
Raman silent Os(III) by FADH2 yields a characteristic enzyme-substrate calibration curve in the
millimolar range. Furthermore, coupling of electronic resonant Raman of the osmium complex with the
SERS amplification by Au NPs plasmon resonance has been demonstrated which leads to an extra
enhancement of the biosensor signal. We present a proof of concept extending the work done with
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
millimolar range. Furthermore, coupling of electronic resonant Raman of the osmium complex with the
SERS amplification by Au NPs plasmon resonance has been demonstrated which leads to an extra
enhancement of the biosensor signal. We present a proof of concept extending the work done with
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
millimolar range. Furthermore, coupling of electronic resonant Raman of the osmium complex with the
SERS amplification by Au NPs plasmon resonance has been demonstrated which leads to an extra
enhancement of the biosensor signal. We present a proof of concept extending the work done with
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
millimolar range. Furthermore, coupling of electronic resonant Raman of the osmium complex with the
SERS amplification by Au NPs plasmon resonance has been demonstrated which leads to an extra
enhancement of the biosensor signal. We present a proof of concept extending the work done with
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
millimolar range. Furthermore, coupling of electronic resonant Raman of the osmium complex with the
SERS amplification by Au NPs plasmon resonance has been demonstrated which leads to an extra
enhancement of the biosensor signal. We present a proof of concept extending the work done with
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
2 yields a characteristic enzyme-substrate calibration curve in the
millimolar range. Furthermore, coupling of electronic resonant Raman of the osmium complex with the
SERS amplification by Au NPs plasmon resonance has been demonstrated which leads to an extra
enhancement of the biosensor signal. We present a proof of concept extending the work done with
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.
We present a proof of concept extending the work done with
planar surfaces to core-shell NPs as an advance in the design of glucose-responsive chemistry detected
by SERS-like methods.