INVESTIGADORES
PALATNIK Javier Fernando
artículos
Título:
Identification of new microRNA-regulated genes by conserved targeting in plant species
Autor/es:
CHOROSTECKI, U; CROSA, V; LODEYRO, A; BOLOGNA, NG; MARTIN, AP; CARRILLO, N; SCHOMMER, C; PALATNIK, J.F.
Revista:
NUCLEIC ACIDS RESEARCH
Editorial:
OXFORD UNIV PRESS
Referencias:
Lugar: Oxford; Año: 2012 vol. doi: p. 1 - 12
ISSN:
0305-1048
Resumen:
MicroRNAs (miRNAs) are major regulators of gene expression in
multicellular organisms. They recognize their targets by sequence
complementarity and guide them to cleavage or translational arrest. It
is generally accepted that plant miRNAs have extensive complementarity
to their targets and their prediction usually relies on the use of
empirical parameters deduced from known miRNA-target interactions. Here,
we developed a strategy to identify miRNA targets which is mainly based
on the conservation of the potential regulation in different species.
We applied the approach to expressed sequence tags datasets from
angiosperms. Using this strategy, we predicted many new interactions and
experimentally validated previously unknown miRNA targets in
Arabidopsis thaliana. Newly identified targets that are broadly
conserved include auxin regulators, transcription factors and
transporters. Some of them might participate in the same pathways as the
targets known before, suggesting that some miRNAs might control
different aspects of a biological process. Furthermore, this approach
can be used to identify targets present in a specific group of species,
and, as a proof of principle, we analyzed Solanaceae-specific targets.
The presented strategy can be used alone or in combination with other
approaches to find miRNA targets in plants.