INVESTIGADORES
PALATNIK Javier Fernando
artículos
Título:
Parallel screening and optimization ofprotein constructs for structural studies
Autor/es:
RASIA, RM; NOIRCLERC-SAVOYE, M; BOLOGNA, NG; GALLET, B; PLEVIN, MJ; PALATNIK, JF; BRUTSCHER, B; VERNET, B; BOISBOUVIER, J
Revista:
PROTEIN SCIENCE
Editorial:
JOHN WILEY & SONS INC
Referencias:
Año: 2009 p. 834 - 839
ISSN:
0961-8368
Resumen:
A major challenge in structural biology remains the identification of protein constructs
amenable to structural characterization. Here, we present a simple method for parallel expression,
labeling, and purification of protein constructs (up to 80 kDa) combined with rapid evaluation by
NMR spectroscopy. Our approach, which is equally applicable for manual or automated
implementation, offers an efficient way to identify and optimize protein constructs for NMR or
X-ray crystallographic investigations.