Partial Substitution of Fishmeal by Meat and Bone Meal, Soybean Meal, and Squid concentrate in Feeds for the Prawn, Artemesia longinaris: Effect on Digestive Proteinases.

FERNÁNDEZ GIMENEZ, ANALIA VERÓNICA; DÍAZ, ANA CRISTINA; VELURTAS, SUSANA MARIA; FENUCCI, JORGE LINO

ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH

ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH

Lugar: Bamidgeh; Año: 2009 vol. 61 p. 48 - 56

0792-156X

Partial Substitution of Fish Meal by Alternative Protein Sources in Feed Formulation for Artemesia longinaris (Decapoda, Penaeidae): Effect of Digestive Proteinases Analía Verónica Fernández Gimenez1,3*, Ana Cristina Díaz2,3, Susana María Velurtas3 and Jorge Lino Fenucci1,3 1Consejo Nacional de Investigaciones Científicas y Técnicas; 2Comisión de Investigaciones Científicas; 3Departamento de Ciencias Marinas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata; Funes 3350, B7602AYL, Mar del Plata, Argentina; fgimenez@mdp.edu.ar Key words: Artemesia longinaris; Chymotrypsin; Digestive proteinases; Enzyme assays; Prawn nutrition; Protein sources; Trypsin Running title: Alternative Protein Sources for Artemesia longinaris (Decapoda, Penaeidae) Abstract The present study describes the effect of different protein sources in formulated feeds on the proteinases activity in the midgut gland of Artemesia longinaris. Three isoproteic formulated feeds were designed. The basal diet contained 48% fish meal and 17% soybean meal as the primary protein sources (D1). The fish meal was partially replaced with meat and bone meal (D2); and D3 with soybean meal and squid protein concentrate. Midgut gland extracts from all treatments and wild prawns (as control group) were assayed to quantify the proteinase activity. Proteinases classes were identified by SDS-PAGE; specific inhibitors were used. Specific proteolytic activity in wild prawn and individuals fed with D2 were higher than other treatments. The trypsin specific activity in individuals fed with formulated feeds (from 1.9±0.27 to 2.6±0.73 abs min-1 mg-1) was higher than observed in wild prawns (0.6±0.60 abs min-1 mg-1). Chymotrypsin activity was higher for prawns fed with D2. Proteinase activity in all samples for azocasein was inhibited by SBTI and TLCK. Six active bands distributed from 16.6 to 53.7 kDa were detected; four trypsins (16.6, 18.2, 21.9, 25.1 kDa) and one chymotrypsin (53.7 kDa) were found. Our findings determined an adaptation of proteolytic activity to the protein quality in feeds and demonstrate that fish meal in feed can be partially replaced by soybean meal in combination with squid protein concentrate. * Author for correspondance