Digestive proteinases of Artemesia longinaris (Decapoda, Penaeidae) and relationship with molting

FERNÁNDEZ GIMENEZ, ANALIA VERÓNICA; GARCÍA CARREÑO FERNANDO LUIS; NAVARRETE DEL TORO MARÍA DE LOS ANGELES; FENUCCI JORGE LINO

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. PART B, BIOCHEMISTRY & MOLECULAR BIOLOGY.

ELSEVIER SCIENCE INC

Año: 2002 vol. 132 p. 593 - 598

1096-4959

Digestive proteinases of Artemesia longinaris (Decapoda, Penaeidae) and relationship with molting Penaeidae) and relationship with molting Artemesia longinaris (Decapoda, Penaeidae) and relationship with molting) and relationship with molting A.V. Fernandez Gimenez , F.L. Garcýa-Carreno *, M.A. Navarrete del Toro , J.L. Fenucci a b, b a ´ ´ ˜a b, b a ´ ´ ˜ Departamento Ciencias Marinas, Facultad Ciencias Exactas y Naturales, CONICET, Universidad Nacional de Mar del Plata, aa Funes 3350, B7602AYL, Mar del Plata, Argentina Centro de Investigaciones Biologicas del Noroeste (CIBNOR), A.P. 129, La Paz, B.C.S, 23000, Mexico b ´(CIBNOR), A.P. 129, La Paz, B.C.S, 23000, Mexico b ´ Received 21 June 2001; received in revised form 17 January 2002; accepted 10 April 2002 Abstract Digestive proteinase activities of Artemesia longinaris were assayed at different stages of the molting cycle. Total proteolytic activity in the hepatopancreas was highest during postmolt. Trypsin and chymotrypsin activities were highest during intermolt. Specific inhibitors and zymograms of A. longinaris hepatopancreas extracts showed four trypsins proteolytic activity in the hepatopancreas was highest during postmolt. Trypsin and chymotrypsin activities were highest during intermolt. Specific inhibitors and zymograms of A. longinaris hepatopancreas extracts showed four trypsins Artemesia longinaris were assayed at different stages of the molting cycle. Total proteolytic activity in the hepatopancreas was highest during postmolt. Trypsin and chymotrypsin activities were highest during intermolt. Specific inhibitors and zymograms of A. longinaris hepatopancreas extracts showed four trypsinsA. longinaris hepatopancreas extracts showed four trypsins (14.79, 15.49, 16.60, 17.38 kDa, respectively) and three chymotrypsins (21.38, 22.91, 27.54 kDa, respectively). Our results suggest that proteolytic activity in the hepatopancreas of A. longinaris is influenced by the molting cycle. Types and activity of prawn digestive enzymes constitute background information to further study the digestive abilities of these organisms and will lead to understanding their nutritional needs and feeding ecology.
2002 Elsevier Science Inc. All rights reserved. Inc. All rights reserved. and activity of prawn digestive enzymes constitute background information to further study the digestive abilities of these organisms and will lead to understanding their nutritional needs and feeding ecology.
2002 Elsevier Science Inc. All rights reserved. Inc. All rights reserved. results suggest that proteolytic activity in the hepatopancreas of A. longinaris is influenced by the molting cycle. Types and activity of prawn digestive enzymes constitute background information to further study the digestive abilities of these organisms and will lead to understanding their nutritional needs and feeding ecology.
2002 Elsevier Science Inc. All rights reserved. Inc. All rights reserved. and activity of prawn digestive enzymes constitute background information to further study the digestive abilities of these organisms and will lead to understanding their nutritional needs and feeding ecology.
2002 Elsevier Science Inc. All rights reserved. Inc. All rights reserved. 14.79, 15.49, 16.60, 17.38 kDa, respectively) and three chymotrypsins (21.38, 22.91, 27.54 kDa, respectively). Our results suggest that proteolytic activity in the hepatopancreas of A. longinaris is influenced by the molting cycle. Types and activity of prawn digestive enzymes constitute background information to further study the digestive abilities of these organisms and will lead to understanding their nutritional needs and feeding ecology.
2002 Elsevier Science Inc. All rights reserved. Inc. All rights reserved. and activity of prawn digestive enzymes constitute background information to further study the digestive abilities of these organisms and will lead to understanding their nutritional needs and feeding ecology.
2002 Elsevier Science Inc. All rights reserved. Inc. All rights reserved. A. longinaris is influenced by the molting cycle. Types and activity of prawn digestive enzymes constitute background information to further study the digestive abilities of these organisms and will lead to understanding their nutritional needs and feeding ecology.
2002 Elsevier Science Inc. All rights reserved. Inc. All rights reserved.
2002 Elsevier Science Inc. All rights reserved. Keywords: Artemesia longinaris; Chymotrypsin; Hepatopancreas; Inhibitors; Molting cycle; Physiology; Prawn; Serine proteinases; Trypsin Trypsin ; Chymotrypsin; Hepatopancreas; Inhibitors; Molting cycle; Physiology; Prawn; Serine proteinases; Trypsin