INVESTIGADORES
SAENZ Daniel Alberto
artículos
Título:
Retinal oxidative stress induced by intraocular pressure.
Autor/es:
MORENO, M.C.; CAMPANELLI, J; SANDE, P; SAENZ, D.; KELLER SARMIENTO, M; ROSENSTEIN, R.
Revista:
FREE RADICAL BIOLOGY AND MEDICINE
Referencias:
Año: 2004 vol. 37 p. 803 - 812
ISSN:
0891-5849
Resumen:
AbstractGlaucoma is an optic neuropathy in which retinal ganglion cells die probably through an apoptotic process.
Apoptosis is known to involve free radicals in several systems including the retina. In this context, the aim of the present
work was to analyze retinal oxidative damage in rats with glaucoma induced by the chronic injection of hyaluronic acid
in the eye anterior chamber. The results showed a significant decrease in total retinal superoxide dismutase and catalase
activities after 6 and 3 weeks of treatment with hyaluronic acid, respectively. Also, although GPX activity increased after
10 weeks of ocular hypertension, GSH levels significantly decreased at 6 weeks of treatment with hyaluronic acid.
Moreover, retinal lipid peroxidation significantly increased in a time-of-hypertension-dependent manner. On the other
hand, a significant decrease in both diurnal and nocturnal retinal melatonin content was detected at 3, 6, or 10 weeks of
treatment with hyaluronic acid. The present results suggest that retinal oxidative stress may be involved in glaucomatous
cell death. Thus, manipulation of intracellular redox status using antioxidants may be a new therapeutic tool to prevent
glaucomatous neurodegeneration.Glaucoma is an optic neuropathy in which retinal ganglion cells die probably through an apoptotic process.
Apoptosis is known to involve free radicals in several systems including the retina. In this context, the aim of the present
work was to analyze retinal oxidative damage in rats with glaucoma induced by the chronic injection of hyaluronic acid
in the eye anterior chamber. The results showed a significant decrease in total retinal superoxide dismutase and catalase
activities after 6 and 3 weeks of treatment with hyaluronic acid, respectively. Also, although GPX activity increased after
10 weeks of ocular hypertension, GSH levels significantly decreased at 6 weeks of treatment with hyaluronic acid.
Moreover, retinal lipid peroxidation significantly increased in a time-of-hypertension-dependent manner. On the other
hand, a significant decrease in both diurnal and nocturnal retinal melatonin content was detected at 3, 6, or 10 weeks of
treatment with hyaluronic acid. The present results suggest that retinal oxidative stress may be involved in glaucomatous
cell death. Thus, manipulation of intracellular redox status using antioxidants may be a new therapeutic tool to prevent
glaucomatous neurodegeneration.