CERZOS   05458
CENTRO DE RECURSOS NATURALES RENOVABLES DE LA ZONA SEMIARIDA
Unidad Ejecutora - UE
artículos
Título:
Hydrogen Peroxide as Chemical sterilizer for Lilium Micropropagation
Autor/es:
NÉSTOR CURVETTO,, PABLO MARINANGELI, GABRIELA MOCKEL
Revista:
Agricell Report
Referencias:
Año: 2007 vol. 48 p. 41 - 42
Resumen:
At Argentina´s Universidad Nacional del Sur and CERZOS, N. Curvetto, P.
Marinangeli, and G. Mockel have compared tradicional micropropagation methods with
a thecnique that uses inexpensive and easily available materials to disifest materials and
culture media, and addition of hydrogen peroxide into the nutrient media to prevent
growth of fungal and bacterial contaminants during micropropagation of Lilium
longiflorum (Biocell 30 (3): 497-500, 2006).
As a traditional method (control), the culture medium was sterilized in an autoclave.
Scale segments were disinfested by treatment with ethanol 70% for one minute
followed by immersion in sodium hypochlorite solution (1.6 grams active chloride per
liter) containing two drops of Tween 20 per liter for 20 minutes, and washed three times
with sterile distilled water, then sliced into explants. In the hydrogen peroxide
technique, the culture medium was sterilized in a home pressure cooker. Scales were
placed in sterile Petri dishes containing a 0.03% hydrogen peroxide solution then cut
into approximately 2 mm transverse sections and placed on nutrient medium containing
hydrogen peroxide at a concentrations of 0.005, 0.010, 0.015 or 0.020% to control
contamination during in vitro establishment and subsequent culture.
Curvetto et al. found that the level of culture contamination was within acceptable
limits in all of the treatments, although it was higher in the hydrogen peroxide
treatments (40%) as compared with the traditional protocols (20%). No significant
differences in the number of bulblets per explant were observed and at the end of the
multiplication phase, bulblets treated with 0.02% hydrogen peroxide showed a greater
biomass as compared with other treatments. These bulblets also had a higher relative
growth ratio with respect to the traditional method when cultured for an additional
period of time and showed the highest average bulblet fresh weight.
The authors conclude that their results suggest that hydrogen peroxide can be used
as a low cost alternative method to traditional micropropagation techniques by hobbysts
or small lily producers to micropropagate L. longiflorum. Hydrogen peroxide up to
0.02% has no deleterious effects on bulblet differentiation, but it does have a
stimulatory effect on bulblet growth. The higher bulblet mass obtained after 18 week
cultivation period in the case of the 0.02% hydrogen peroxide treatment would
eventually result in a better plantlet performance growth ex vitro.Lilium
longiflorum (Biocell 30 (3): 497-500, 2006).
As a traditional method (control), the culture medium was sterilized in an autoclave.
Scale segments were disinfested by treatment with ethanol 70% for one minute
followed by immersion in sodium hypochlorite solution (1.6 grams active chloride per
liter) containing two drops of Tween 20 per liter for 20 minutes, and washed three times
with sterile distilled water, then sliced into explants. In the hydrogen peroxide
technique, the culture medium was sterilized in a home pressure cooker. Scales were
placed in sterile Petri dishes containing a 0.03% hydrogen peroxide solution then cut
into approximately 2 mm transverse sections and placed on nutrient medium containing
hydrogen peroxide at a concentrations of 0.005, 0.010, 0.015 or 0.020% to control
contamination during in vitro establishment and subsequent culture.
Curvetto et al. found that the level of culture contamination was within acceptable
limits in all of the treatments, although it was higher in the hydrogen peroxide
treatments (40%) as compared with the traditional protocols (20%). No significant
differences in the number of bulblets per explant were observed and at the end of the
multiplication phase, bulblets treated with 0.02% hydrogen peroxide showed a greater
biomass as compared with other treatments. These bulblets also had a higher relative
growth ratio with respect to the traditional method when cultured for an additional
period of time and showed the highest average bulblet fresh weight.
The authors conclude that their results suggest that hydrogen peroxide can be used
as a low cost alternative method to traditional micropropagation techniques by hobbysts
or small lily producers to micropropagate L. longiflorum. Hydrogen peroxide up to
0.02% has no deleterious effects on bulblet differentiation, but it does have a
stimulatory effect on bulblet growth. The higher bulblet mass obtained after 18 week
cultivation period in the case of the 0.02% hydrogen peroxide treatment would
eventually result in a better plantlet performance growth ex vitro.(Biocell 30 (3): 497-500, 2006).
As a traditional method (control), the culture medium was sterilized in an autoclave.
Scale segments were disinfested by treatment with ethanol 70% for one minute
followed by immersion in sodium hypochlorite solution (1.6 grams active chloride per
liter) containing two drops of Tween 20 per liter for 20 minutes, and washed three times
with sterile distilled water, then sliced into explants. In the hydrogen peroxide
technique, the culture medium was sterilized in a home pressure cooker. Scales were
placed in sterile Petri dishes containing a 0.03% hydrogen peroxide solution then cut
into approximately 2 mm transverse sections and placed on nutrient medium containing
hydrogen peroxide at a concentrations of 0.005, 0.010, 0.015 or 0.020% to control
contamination during in vitro establishment and subsequent culture.
Curvetto et al. found that the level of culture contamination was within acceptable
limits in all of the treatments, although it was higher in the hydrogen peroxide
treatments (40%) as compared with the traditional protocols (20%). No significant
differences in the number of bulblets per explant were observed and at the end of the
multiplication phase, bulblets treated with 0.02% hydrogen peroxide showed a greater
biomass as compared with other treatments. These bulblets also had a higher relative
growth ratio with respect to the traditional method when cultured for an additional
period of time and showed the highest average bulblet fresh weight.
The authors conclude that their results suggest that hydrogen peroxide can be used
as a low cost alternative method to traditional micropropagation techniques by hobbysts
or small lily producers to micropropagate L. longiflorum. Hydrogen peroxide up to
0.02% has no deleterious effects on bulblet differentiation, but it does have a
stimulatory effect on bulblet growth. The higher bulblet mass obtained after 18 week
cultivation period in the case of the 0.02% hydrogen peroxide treatment would
eventually result in a better plantlet performance growth ex vitro.et al. found that the level of culture contamination was within acceptable
limits in all of the treatments, although it was higher in the hydrogen peroxide
treatments (40%) as compared with the traditional protocols (20%). No significant
differences in the number of bulblets per explant were observed and at the end of the
multiplication phase, bulblets treated with 0.02% hydrogen peroxide showed a greater
biomass as compared with other treatments. These bulblets also had a higher relative
growth ratio with respect to the traditional method when cultured for an additional
period of time and showed the highest average bulblet fresh weight.
The authors conclude that their results suggest that hydrogen peroxide can be used
as a low cost alternative method to traditional micropropagation techniques by hobbysts
or small lily producers to micropropagate L. longiflorum. Hydrogen peroxide up to
0.02% has no deleterious effects on bulblet differentiation, but it does have a
stimulatory effect on bulblet growth. The higher bulblet mass obtained after 18 week
cultivation period in the case of the 0.02% hydrogen peroxide treatment would
eventually result in a better plantlet performance growth ex vitro.L. longiflorum. Hydrogen peroxide up to
0.02% has no deleterious effects on bulblet differentiation, but it does have a
stimulatory effect on bulblet growth. The higher bulblet mass obtained after 18 week
cultivation period in the case of the 0.02% hydrogen peroxide treatment would
eventually result in a better plantlet performance growth ex vitro.ex vitro.