INVESTIGADORES
GONZALEZ FLECHA Francisco Luis
artículos
Título:
Labeling of Proteins with Fluorescent Probes. PHOTOPHYSICAL CHARACTERIZATION OF DANSYLATED BOVINE SERUM ALBUMIN
Autor/es:
VALERIA LEVI; F. LUIS GONZÁLEZ-FLECHA
Revista:
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION
Editorial:
The International Union of Biochemistry and Molecular Biology
Referencias:
Año: 2003 vol. 31 p. 333 - 336
ISSN:
1470-8175
Resumen:
Fluorescence spectroscopy is a widely used technique in biophysical studies. One of the strategiesfrequently used consists of labeling biomolecules with fluorescent probes, which have distinctive photo-physical properties. This methodology allows the study of a wide variety of structural features of thebiomolecule. We describe a simple laboratory activity for undergraduate Biophysical Chemistry courses.The experimental work includes two activities: labeling BSA with dansyl chloride and analyzing the resultingabsorption and fluorescence spectra. The discussion of these activities helps students to understand thebasis of fluorescence spectroscopy with emphasis in the application to biological systems.
