α-lipoic acid protects kidney from oxidative stress and mitochondrial function associated to inflammatory conditions

CIMOLAI MARÍA CECILIA *; VANASCO VIRGINIA *(*EQUALLY CONTRIBUTED); MARCHINI TIMOTEO; MAGNANI NATALIA D.; EVELSON PABLO; ALVAREZ SILVIA

Food and Function

Cambridge : Royal Society of Chemistry

Año: 2014 vol. 5 p. 3143 - 3150

2042-6496

An adequate redox status is important for maintaining mitochondrial function in inflammatory conditions. The aim of this work was to evaluate the effects of α-lipoic acid (LA) in kidney oxidative metabolism and mitochondrial function in lipopolysaccharide (LPS) treated rats. Sprague-Dawley rats (female, 45 ± 5 days old) were treated with LPS (10 mg/kg) and/or LA (100 mg/kg). LA prevented the 1.2 fold increase in NO production, decreased (30-40%) mitochondrial complex I-III and IV activities, and decreased (26%) membrane potential and cardiolipin oxidation (76%) observed in LPS-treated animals. No differences were observed in mitochondrial O2 consumption, mitochondrial complex II-III activity, and ATP production when LPS group was compared to LA+LPS group. Based on the improvement of the mitochondrial function, decreased production of mitochondrial NO and restoration of cardiolipin levels, this work provides new evidence that α-lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction.