INVESTIGADORES
GALLEANO Monica Liliana
artículos
Título:
Nitric oxide and iron overload. Limitations of ESR detection by DETC
Autor/es:
GALLEANO M; AIMO L; BORRONI MV; PUNTARULO S
Revista:
TOXICOLOGY
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Amsterdam; Año: 2001 vol. 167 p. 199 - 205
ISSN:
0300-483X
Resumen:
The ability of the ESR technique based on diethyldithiocarbamate (DETC)
administration was studied as a suitable method to assess NO generation
in vivo. The technique was successfully employed to measure NO
generation after LPS treatment. DETC2-Fe-NO adducts were detected in
liver homogenates of iron overloaded animals. When iron was administered
to the animals simultaneously with LPS, NO-dependent signal increased
122%, but the content of NO2- and NO3- in sera was significantly lower
(44%) as compared to LPS-treated rats. Iron dextran administration was
responsible for a three-fold increase in the DETC2-Fe-NO content in
non-LPS treated rats, while NOS activity and sera NO2- and NO3- levels
remained unaffected. The adduct generation rate by a chemical NO-source
was recorded in the presence of either control or iron overloaded
homogenates supplemented with DETC in vivo. The exposure of liver
homogenates to NO was performed either by the addition of 1 mM SNAP as
NO donor or infusing an aqueous NO solution. In the presence of iron
overloaded samples the adduct generation rate was 3.8-4.4-fold higher
than in the presence of control samples. This effect restricts the
applicability of the method to experimental conditions where iron levels
remain constant, therefore it is not suitable for NO generation studies
in experimental models where animals were subjected to iron overload.