IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
artículos
Título:
Reporter assays
Autor/es:
MF CERIANI
Revista:
METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.)
Referencias:
Año: 2007 vol. 362 p. 455 - 463
ISSN:
1064-3745
Resumen:
Transcriptional feedback loops are at the core of the molecular clockworks. As single clock genes were cloned it was compelling to develop an assay that allowed simple and direct functional testing of putative activators or repressors of transcription. This chapter includes a general description and guidelines to carry out transcriptional assays in transiently transfected Schneider´s cells.