Candida dubliniensis and Candida albicans discrimination by colony morphotype in Sabouraud-Triphenyltetrazolium Agar

GAMARRA, SOLEDAD; MANCILLA, ESTEFANÍA; DUDIUK, CATIANA; GARCIA, GUILLERMO

REVISTA IBEROAMERICANA DE MICOLOGIA

ASOCIACION ESPANOLA MICOLOGIA-AEM

Lugar: Londres; Año: 2014

1130-1406

Background: Candida dubliniensis is a germ tube and chlamydoconidia producing Candida species that may be misidentified as C. albicans. Molecular-based methods are the most reliable techniques for C. albicans and C. dubliniensis differentiation. However, accurate, quick and inexpensive phenotypic tests are needed to be used in low-complexity mycology laboratories. Objective: To evaluate colony morphotype on Sabouraud-triphenyltetrazolium agar as a tool for C. dubliniensis and C. albicans differentiation. Methods: The morphology of 126 strains C. albicans and C. dubliniensis strains were evaluated and compared with identification by molecular methods. Results and conclusion: The method showed 100% sensitivity and specificity when color and the presence or absence of large white mycelial halo was evaluated.