INVESTIGADORES
REGENTE Mariana Clelia
artículos
Título:
Phosphorylated 11S globulins in sunflower seeds
Autor/es:
IVANA QUIROGA (PRIMER AUTOR); MARIANA REGENTE (PRIMER AUTOR); LUCIANA PAGNUSSAT; ANA MALDONADO; JESÚS JORRÍN; LAURA DE LA CANAL
Revista:
SEED SCIENCE RESEARCH
Editorial:
CAMBRIDGE UNIV PRESS
Referencias:
Lugar: Cambridge; Año: 2013 vol. 23 p. 199 - 204
ISSN:
0960-2585
Resumen:
Helianthinins are storage proteins present in Helianthus annuus seeds belonging to the 11S globulin family. We describe here that a fraction of the helianthinins is phosphorylated. This conclusion is supported by different criteria including identification by MALDI-TOF of major protein bands revealed with a specific dye for phosphoproteins, anti-phosphoserine antibody and binding to a phosphoprotein affinity matrix. Moreover, we show that the phosphorylation status of helianthinins changes following germination. Helianthinins are storage proteins present in Helianthus annuus seeds belonging to the 11S globulin family. We describe here that a fraction of the helianthinins is phosphorylated. This conclusion is supported by different criteria including identification by MALDI-TOF of major protein bands revealed with a specific dye for phosphoproteins, anti-phosphoserine antibody and binding to a phosphoprotein affinity matrix. Moreover, we show that the phosphorylation status of helianthinins changes following germination.
