A DNA vaccine coding for the quimera BLSOmp31 induced a better degree of protection against B. ovis and a similar degree of protection against B. melitensis than Rev.1 vaccination

CASSATARO, J., PASQUEVICH, K. A., ESTEIN, S. M. , LAPLAGNE, D., VELIKOVSKY, C. A., DE LA BARRERA, S., BOWDEN, R. A., FOSSATI, C. A., GIAMBARTOLOMEI, G. H. AND GOLDBAUM, F. A.

VACCINE

Año: 2007 vol. 25 p. 5958 - 5967

0264-410X

In the present study, we reported an attempt to improve the immunogenicity and protective capacity of the chimera BLSOmp31 usinga different antigen delivery: DNA vaccination. Vaccination of BALB/c mice with the DNA vaccine coding for the chimera BLSOmp31(pCIBLSOmp31) provided the best protection level against Brucella ovis, which was significantly higher than the given by the co-deliveryof both plasmids coding for the whole proteins (pcDNABLS + pCIOmp31) and even higher than the control vaccine Rev.1. Moreover,pCIBLSOmp31 induced higher protection against Brucella melitensis than pcDNABLS + pCIOmp31 but similar protection than Rev.1. Thechimera induced a strong humoral response against the inserted peptide. It also induced peptide- and BLS-specific cytotoxic T responses.The insertion of this peptide on BLS induced stronger T helper 1 responses specific for the carrier (BLS), thus our results represent a case ofsynergic strengthening between two Brucella antigens. Hitherto, this is the first indication that a recombinant subunit vaccine elicits greaterprotection than whole Brucella.