INVESTIGADORES
SÁNCHEZ SebastiÁn
artículos
Título:
Neuroendocrine system of the digestive tract in Rhamdia quelen juvenile: an immunohistochemical study
Autor/es:
HERNÁNDEZ, D.R.; VIGLIANO, F.A.; SÁNCHEZ, SEBASTIÁN; BERMÚDEZ, R.; DOMITROVIC, H.A.; QUIROGA, M.I.
Revista:
TISSUE & CELL
Editorial:
CHURCHILL LIVINGSTONE
Referencias:
Año: 2012 vol. 44 p. 220 - 226
ISSN:
0040-8166
Resumen:
In this work, an immunohistochemical study was performed to determine the distribution and relative frequencies of some neuromodulators of the digestive tract of silver catfish (Rhamdia quelen). The digestive tract of silver catfish was divided into six portions; the oesophagus, stomach, intestine (ascendant, descendant and convoluted segments), and rectum. Immunohistochemical method using a pool of specific antisera against-gastrin, -cholecystokinin-8, -leu-enkephalin, -neuropeptide Y, -calcitonin gene-related peptide (CGRP), and -vasoactive intestinal peptide (VIP) was employed. Immunoreactivity to all antisera was identified in neuroendocrine cells (NECs) localized in the gut epithelium, although no reaction was observed in the oesophagus or stomach. The morphology of NECs immunopositive to each antibody was similar. They were slender in shape, with basally located nucleus, and their main axis perpendicular to the basement membrane. The number of NECs immunoreactive to all antisera was higher in the ascendant and descendant intestine, exhibiting a decreasing trend toward distal segments of the gut. In addition, immunoreactivity to CGRP and VIP was observed in the myenteric plexus and nerve fibers distributed in the mucosal, submucosal and muscular layers. The higher number of immunopositive NECs in the ascendant and descendant intestine may indicate the primary role of these segments in the control of food intake by means of orexigenic and anorexigenic peripheral signals.