INVESTIGADORES
CYMERYNG Cora Beatriz
artículos
Título:
Oxaloacetate decarboxylase activities in epimastigotes of Trypanosoma cruzi
Autor/es:
CYMERYNG, CORA; CAZZULO, JUAN JOSÉ; CANNATA, JJB
Revista:
Journal of the Argentine Chemical Society
Editorial:
Asociación Química Argentina
Referencias:
Lugar: Buenos Aires; Año: 1992 vol. 80 p. 1 - 3
ISSN:
0365-0375
Resumen:
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Epimastigotes of Trypanosoma cruzi contain two oxaloacetate-decarboxylating activites (OAD). One is an intrinsic aactivity of the phosphoenolpyruvate carboxykinase (PEPCK), shown by the purified enzyme. The other OAD activity corresponds to a protein which can be separates from PEPCK by ion exchange chromatography on Mono Q or by affinity chromatography on ATP-agarose, and shows distinct properties in terms of divalent cation activation and pH optima. Extraction from cells broken by freezing and thawing affords enzyme preparations rich in Cd2+-activated OAD activity and poor in PEPCK, which can be used as a source for the purification of the former enzyme.
