INVESTIGADORES
CYMERYNG Cora Beatriz
artículos
Título:
ACTH-dependent proteolytic activity of a novel phosphoprotein (p43) intermediary in the activation of arachidonic acid release and steroidogenesis
Autor/es:
CYMERYNG, CORA BEATRIZ; PAZ, C; DADA, L; CORNEJO MACIEL, F; NEUMAN, I; MENDEZ, C; MELE, P; FINKIELSTEIN, C; SOLANO, A; PARK, M; FISCHER, W; TOWBIN, H; SCARTAZZINI, R; PODESTÁ, EJ
Revista:
ENDOCRINE RESEARCH
Editorial:
TAYLOR & FRANCIS INC
Referencias:
Año: 1995 vol. 21 p. 281 - 288
ISSN:
0743-5800
Resumen:
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Arachidonic acid (AA) and the lipooxygenase products have been shown to play an obligatory role in the mechanism of action of LH and ACTH, at a point after cAMP-dependent phosphorylation. We have demonstrated the presence of a phosphoprotein (p43) that responds to cAMP signals to induce steroid synthesis in adrenocortical tissue, an effect that is blocked by phospholipase A2 inhibitors. In this report we demonstrate that p43 exhibits autoproteolytic activity that is regulated by ACTH. Protein purified from ACTH-treated animals exhibited degradation in some of the isoforms resolved on two dimensional gel electrophoresis. Proteinase inhibitors (PMSF and 1,10 phenantroline) inhibited steroid synthesis induced by ACTH and 8-Br-cAMP in intact cells. Addition of exogenous AA reverted in part that inhibition. Here we present evidence for a hormone-regulated proteolytic activity of p43 and for the inhibition of steroidogenesis by proteinase inhibitors acting prior to the release of arachidonic acid.
