Negligible effects of tryptophan on the aflatoxin adsorption of sodium bentonite

A. P. MAGNOLI; P. COPIA; M.P. MONGE; C. E.MAGNOLI; A. M. DALCERO; S.M. CHIACCHIERA

FOOD ADDITIVES AND CONTAMINANTS

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2014

0265-203X

The main objective of this study was to determine if the competitive adsorption of tryptophan (Trp) and aflatoxin B1 (AFB1) could potentially affect the ability of a sodium bentonite (NaB) to prevent aflatoxicosis in monogastric animals. The adsorption of Trp and AFB1 on this adsorbent is fast and could be operating on the same time-scale making competition feasible. In vitro competitive adsorption experiments under simulated gastrointestinal conditions were performed. A high affinity of the clay for Trp and NaB was observed. The effect of an excess of KCl to mimic the ionic strength of the physiological conditions were also investigated. A six times decrease in the Trp surface excess at saturation was observed. A similar behavior was previously found for AFB1 adsorption. Taking into account the amount of Trp adsorbed Downloaded by [Ondokuz Mayis Universitesine] at 05:05 12 November 2014 Accepted Manuscript 2 by the clay and the usual adsorbent supplementation level in diets, a decrease in Trp bioavailability is not expected to occur. Tryptophan adsorption isotherms on NaB were Sshaped and were adjusted by the Frumkin-Fowler-Guggenheim model. The reversibility of the adsorption processes was investigated in order to check a potential decrease in the ability of NaB to protect birds against chronic aflatoxicoses. Adsorption processes were completely reversible for Trp, while almost irreversible for AFB1. In spite of the high affinity of the NaB for Trp, probably due to the reversible character of Trp adsorption, no changes in the AFB1 adsorption isotherm were observed when an excess of the amino acid was added to the adsorption medium. As a consequence of the preferential and irreversible AFB1 adsorption and the reversible weak binding of Trp to the NaB, no changes in the aflatoxin sorption ability of the clay are expected to occur in the gastrointestinal tract of birds.