INVESTIGADORES
GÜERCI Alba Mabel
artículos
Título:
Genetic instability induced by low doses of x-rays in hamster cells.
Autor/es:
8. A.I. SEOANE, GÜERCI A.M, AND F.N. DULOUT.
Revista:
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
Editorial:
Taylor and Francis
Referencias:
Lugar: London; Año: 2007 vol. 83 p. 81 - 87
ISSN:
0955-3002
Resumen:
Purpose: Genomic instability involves time delayed events and can be manifested as elevated rates of heritable changes in
the progeny of irradiated cells. To study the induction of chromosomal instability by very low doses of radiation Chinese
Hamster Ovary (CHO) cells were exposed to 10 50 milisieverts (mSv) (10 50 miligrays (mGy)) of x-rays.Genomic instability involves time delayed events and can be manifested as elevated rates of heritable changes in
the progeny of irradiated cells. To study the induction of chromosomal instability by very low doses of radiation Chinese
Hamster Ovary (CHO) cells were exposed to 10 50 milisieverts (mSv) (10 50 miligrays (mGy)) of x-rays.
Materials and methods: Control and irradiated cell populations were assayed for chromosomal aberrations and assessed
using a micronucleus test and anaphase-telophase analysis at the first cell division post-irradiation and at every four
population doublings thereafter up to 16 population doublings post-irradiation.Control and irradiated cell populations were assayed for chromosomal aberrations and assessed
using a micronucleus test and anaphase-telophase analysis at the first cell division post-irradiation and at every four
population doublings thereafter up to 16 population doublings post-irradiation.
Results: Frequencies of micronuclei, anaphase-telophase alterations and chromosomal aberrations were increased when the
cells were analysed immediately after x-ray exposure. Micronuclei and anaphase-telophase alterations showed significantly
increased frequencies when they were analysed at 12 and 16 population doublings after exposure to 50 mSv. Chromosomal
aberrations increased significantly at 12 and 16 population doublings after exposure to 10 mSv and 50 mSv.Frequencies of micronuclei, anaphase-telophase alterations and chromosomal aberrations were increased when the
cells were analysed immediately after x-ray exposure. Micronuclei and anaphase-telophase alterations showed significantly
increased frequencies when they were analysed at 12 and 16 population doublings after exposure to 50 mSv. Chromosomal
aberrations increased significantly at 12 and 16 population doublings after exposure to 10 mSv and 50 mSv.
Conclusions: Our results are consistent with the presence of a phenomenon by which the initial DNA damage in the
surviving cells is memorized. Micronuclei and achromatic lessions were the main cytogenetic damage observed in cells
exposed to very low doses of x-rays, indicating that these low doses are able to induce genetic instability.Our results are consistent with the presence of a phenomenon by which the initial DNA damage in the
surviving cells is memorized. Micronuclei and achromatic lessions were the main cytogenetic damage observed in cells
exposed to very low doses of x-rays, indicating that these low doses are able to induce genetic instability.