Presence of neutrophil bearing antigen in lymphoid organs of immune mice

BELKYS ANGÉLICA MALETTO, ANDREA SILVANA RÓPOLO, DIEGO OSCAR ALIGNANI, MIRIAM VERÓNICA LISCOVSKY, ROMINA PAOLA RANOCCHIA, VÍCTOR GABRIEL MORÓN AND MARÍA CRISTINA PISTORESI-PALENCIA

BLOOD

The American Society of Hematology

Año: 2006 p. 3094 - 3102

Neutrophils play a crucial early role during the innate response, but little is known about their possible contribution when an adaptative immune response is installed. A robust neutrophilia and a Th1 immune response are present after immunization with Complete Freund’s Adjuvant (CFA). We show that when FITC-labeled OVA was injected into the footpad of OVA/CFA immunized mice, the main OVA-FITC+ cells recruited in draining popliteal lymph nodes (LN) were neutrophils, with most of them arriving at the LN by means of lymphatic vessels. The development of this OVA-FITC+ neutrophil influx requires an immune response against OVA. The OVA-FITC+ neutrophils present in LN displayed mainly intracellular TNF-a and their depletion resulted in an increase in the specific IL-5 levels. These data provide new evidence about the role played by neutrophils in vivo in adaptative immunity.