IBBM 21076
INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
artículos
Título:
Novel tnpR-based transposable promoter traps suitable for RIVET studies in different Gram-negative bacteria.
Autor/es:
LOZANO, MJ.; SALAS, ME.; GIUSTI, MA.; MARTINI, MC.; LOPEZ, JL.; SALTO, IP.; DEL PAPA, MF.; PISTORIO, M.; LAGARES, A.
Revista:
JOURNAL OF MICROBIOLOGICAL METHODS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2013 vol. 93 p. 9 - 11
ISSN:
0167-7012
Resumen:
The preparation of plasmid-borne RIVET libraries can be troublesome when high genomic coverages are needed. We present here the construction and functional validation of a new set of miniTn5 promoter traps to generate tnpR-based RIVET libraries. The ability to generate tnpR transcriptional fusions by transposition will significantly facilitate the setup of RIVET studies in those bacteria where Tn5 transposition is operative.
