A mechanistic molecular test of the plant-sanction hypothesis in legume-rhizobia mutualism

DIANA MARCO; REBECA PÉREZ-ARNEDO; ÁNGELES HIDALGO-PEREA; JOSÉ OLIVARES; JOSÉ E. RUIZ-SAINZ; JUAN SANJUÁN

ACTA OECOLOGICA-INTERNATIONAL JOURNAL OF ECOLOGY

GAUTHIER-VILLARS/EDITIONS ELSEVIER

Año: 2009 vol. 35 p. 664 - 667

1146-609X

The origin and persistence of mutualism is difficult to explain because of the widespread occurrence of exploitative, ?cheating? partners. As a policing strategy stabilising intraspecific cooperation, host sanctions against non N2 fixing, cheating symbionts have been proposed to stabilise mutualism in legume-rhizobium symbiosis. Mechanism of penalisations would include decreased nodular rhizobial viability and/or early nodule senescence. We tested these potential mechanisms of penalisations in split-root experiments using two soybean varieties and two rhizobial strains, a cooperative, normal N2 fixing strain and an isogenic non-fixing derivative. We found no differences in the number of viable rhizobia recovered from nodules and no differential expression of a nodular senescence molecular marker. Thus, our results do not support the hypothesis of plant sanctions acting against cheating rhizobia in our experimental conditions.