IMMUNOLOCALIZATION OF P-GLYCOPROTEIN IN HELMINTHS OF VETERINARY INTEREST

GUERRERO M.; SOLANA H.; ALZOLA R.

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2013 vol. 37 p. 1 - 12

0327-9545

Liver fluke (Fasciola hepatica), Paramphystomum spp, Ascaris spp.and Moniezia spp are endoparasites of herbivores polygastric. Theseworms have molecular and biochemical mechanism of detoxificationfor protection against various xenobiotics includes theanthelmintics. Within the detoxification mechanisms include theuse of extrusion pumps such as P-glycoprotein (P-gp), a membranetransporter protein, which acts secreting a variety of endogenoussubstances and xenobiotics from inside the cell to the extracellularspace via an ATP-dependent mechanism. The mammalian P-gp islocated in cells involved in the absorption, distribution and excretionof drugs such as certain cells of the liver, intestine, blood brainbarrier, among others. This location suggests that P-gp plays animportant role regulating the transport of drugs across cell membranes,altering the kinetic behavior and the bioavailability of drugs.The aim of this study was to identify by immunohistochemistry thepresence and distribution of P-gp in histological sections of theparasites mentioned above. Techniques were performed for routinehistological cuts of parasites and proceeded to performimmunolocalization with P glycoprotein (monoclonal antibodySigma) and immunohistochemistry using LSAB (DAKO Lab).The results showed immunolocalization of P-gp in vitellogenic cellsand spicules of Fasciola hepatica, in cuticle of Ascaris and Monieziaspp, not immunolocalized in sections of Paramphystomum spp.