Development and validation of a micromethod for fast quantification of 5-n-alkylresorcinols in grains and whole grain products

SAMPIETRO, D.A.; APUD, G.R.; JIMENEZ, C.M.; BELIZÁN, M.M.E; VATTUONE, M.A.; CATALAN, C. A. N,

FOOD CHEMISTRY

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2013 vol. 141 p. 3546 - 3551

0308-8146

A 96-well plate micromethod was developed to measure 5-n-alkylresorcinols(5nARs)  in cereal grains and food derived products.The 5nARs reacted in alkaline alcoholic medium with Fast Blue RR ½ZnCl2 salt to yield coloured azo-derivatives.The highest sensitivity for  5nARs was obtained at 490 nm with 0.025% ethanolic Fast Blue RR and 5% K2CO3. This reaction showed good lineality for olivetol (0.05-0.20 μg). Contents of 5nARs determined in cereal grains and derived products by the new Fast Blue RR micromethod were highly correlated (R2=0.9944) with those obtained by a Fast Blue B method currently used.A  Bland-Altman analysis indicated a small positive bias near to zero (R2=0.0401), suggesting that the methods can be interchangeably used. The new reaction is completed in 15 minutes and the colored products are read within the 15 minutes after completion.The micromethod offers a fast analysis of 5nARs in cereal grains and derived products with low consumption of reagents and solvents.