INVESTIGADORES
KAUFMAN Teodoro Saul
artículos
Título:
Chemometric Determination of Amiloride Hydrochloride, Atenolol, Hydrochlorothiazide and Timolol Maleate in Synthetic Mixtures and Pharmaceutical Formulations
Autor/es:
MÓNICA C. F. FERRARO; PATRICIA M. CASTELLANO; TEODORO S. KAUFMAN
Revista:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Editorial:
Elsevier
Referencias:
Lugar: Amsterdam; Año: 2004 vol. 34 p. 305 - 341
ISSN:
0731-7085
Resumen:
Different
chemometric methods such as classical least squares (CLS), principal components
regression (PCR) and partial least squares with one dependent variable (PLS-1)
applied on UV spectral data (0D) and on their
first derivatives (1D) were evaluated
for the simultaneous quantification of samples containing mixtures of amiloride
hydrochloride, atenolol, hydrochlorothiazide and timolol maleate. Their
performances were compared by means of ANOVA tests, which evidenced that 0D-PCR, 0D-PLS-1, 1D-PCR and 1D-PLS-1 were
reproducible and gave statistically similar results, while 0D-CLS and 1D-CLS displayed
higher variances than the former and failed to comply with the Levene?s
variance homogeneity test at different stages of the method comparison and
validation process. The four statistically equivalent procedures were
successfully applied to the analysis of synthetic samples with two to four
analytes and to commercial tablet preparations containing amiloride
hydrochloride and hydrochlorothiazide alone or in association with atenolol or
timolol maleate.