IIBBA 05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
artículos
Título:
Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia Coli
Autor/es:
TRAGLIA G.; DAVIES C.; FUXMAN BASS J.I.; SOLER BISTUE A. ; ZORREGUIETA A. ; RAMIREZ M.S.; TOLMASKY M.E.
Revista:
BioResearch Open Access
Editorial:
Mary Ann Liebert
Referencias:
Año: 2012 p. 260 - 263
ISSN:
2164-7860
Resumen:
Delivery inside the cells is essential for practical application of antisense technologies. The hybrid locked nucleic acid (LNA)/DNA CAAGTACTGTTCCACCA(LNA residues are underlined) was labeled by conjugation to Alexa Fluor 488 (fLNA/DNA) and tested to determine its ability to penetrate Escherichia coli cells and reach the cyto- plasm. Flow cytometry analysis showed that the fLNA/DNA was associated with 14% of cells from a stationary phase culture, while association with a labeled isosequential oligodeoxynucleotide was negligible. Laser scanning confocal microscopy confirmed that the fLNA/DNA was located inside the cytoplasm.
