Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris

MELISA JACOBS, SILVINA R. SALINAS, MARı´A I. BIANCO AND LUIS IELPI

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2012 vol. 68 p. 1255 - 1258

1744-3091

GumB is a predicted outer membrane lipoprotein that is involved in the synthesis and/or secretion of xanthan gum. This exopolysaccharide, produced by Xanthomonas campestris, is valuable in industry because of its important rheological properties. Solution of the GumB structure will provide insight into the polymerization and/or secretion mechanisms of xanthan gum. GumB was overexpressed and purified and diffraction-quality crystals of native GumB were obtained. A complete data set was collected to 2.54 A resolution with an Rp.i.m.of 0.034. The crystals belonged to space group P212121, with unit-cell parametersa = 84.4, b = 90.5, c = 120.7 A.