RIMAPS and Variogram analysis of Barley Leaf Surfaces

FAVRET, E. AND FUENTES, N.

Microscopy Today

Microscopy Society of America

Lugar: Largo, USA; Año: 2004 vol. 12 p. 24 - 26

It is a common and extended practice the use of microscopic images to simply describe the differences observed on plant tissues. In all cases the images illustrate the taxonomic characteristics of studied species. In this work we introduce a quantitative analysis from digitized images obtained with scanning electron microscopy (SEM) of barley leaf surfaces. The topography of the leaf surfaces of a narrow-leaf mutant and its wild type mother line was characterized, see figure 1, using the Rotated Image with Maximum Average Power Spectrum (RIMAPS) technique and the Variogram method. Spectra resulting from RIMAPS analysis allow to identify  the specimens and to distinguish between the adaxial or the abaxial side of the leaf. These results are complemented obtaining the typical scale lengths that characterize the abaxial surface of both the mutant and the mother line barley leaves. Comparison between the widths of long cells on the two specimens brings out to light the existence of the Golden Ratio value of 1.618. The same value is verified when making the ratio between the width of mother line leaf and the mutant barley.