Toxins in the freshwaters cyanobacterium Cylindroespermosis raciborskii, isolated in Tabocas reservoir in Caruaru. Pernambuco. Brasil.

MOLICA R., ONODERA H., GARCÍA C., RIVAS M., ANDRINOLO D., NACIMIENTO S., MEGURO H., OSHIMA Y., AZEVEDO S., LAGOS N.

PHYCOLOGIA

INT PHYCOLOGICAL SOC

Año: 2002 vol. 41 p. 606 - 611

0031-8884

Cyanobacteria can prrxlucc biotoxins üat are significant hazards to humans. After the in¡oxication incidcnt i¡¡ 1996 at thc city of Caruaru; Bn¡zil, a phyroplankron-monitoring programme r+´as establishcd at its main water supply. thc Tabocas rcservoir. D¿rt¿ obtained during 1997 and 1998 rcvcalcd the dominant spccics at ´lhtncas to be Cylindxtspennapsb ruciáors* ii, rvhich was responsible for a ¡nassivc bloom observed in July-Octob€r 1998. Laboratory cultures of isolatc [TEP- 018 demonstrated highly toxic propc..rties,m icc inoculated with this strain exhibited üre somc synrptoms as tlosc of paral¡ic shcllfrsh poisoning, with an acute lethal cffect of 9.3 mousc units mg-r of dry cells. Several saxitoxin analogues werc idcnriñed in thcsc culturcs, specilicall¡ suxit<¡xin (3.3 mol% total toxin corrtc¡rt). Er)nyautoxin ó (6.4 mol%). der:urbamoylsaxitnxin (1t.5 mol%). neosaxitoxin (l?.1 mol%), and a ncw saxitoxin analogue, which pmvcd to be üte major prtxluct of thc ´Iatxrcas struin. ¡ccr¡unting for 64.6 mol% of the toxin prcscnt in the sample anal¡;scd. Additionally, tlccarbumoytneos ¿riroxin was deiected by liquid chruruatography-rnass spectromctry. Cl,lindro.tpcrmop.sis raciborskíi strain ITEP-OI8 thus prrxluces at leusi lrvc saxitoxin analogucs, including tlre most toxic <¡nesa s a.ssessedb y tnousc bioassa