22- Impact of chronic low-dose alcohol ingestion during sexual maturation of female mice on in-vitro and in-vivo embryo development

E CEBRAL; RETTORI, V; GIMENO, M.A.F

REPRODUCTIVE TOXICOLOGY (ELMSFORD, N.Y.)

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2001 vol. 15 p. 123 - 129

0890-6238

Little is known of the consequences of ethanol intake prior to fertilization on preimplantation embryo development. Recently we showed that chronic 10 and 5% w/v ethanol intake by young female mice reduces in vitro fertilization (IVF) rates. The purpose of the present work was to investigate whether the adverse effects of preconceptional low-dose chronic ethanol intake by sexually maturing female mice affects preimplantation embryo growth in vitro or in vivo in subsequent pregnancy. Prepubertal female mice were given 5% ethanol in their drinking water for 30 days. On day 27 and 29 of the ethanol treatment, females were superovulated. IVF-derived cultured embryos (in vitro development) or embryos obtained from oviducts and uteri (in vivo development) were evaluated. Whether analyzed on a per embryo or per dam basis, ethanol treatment was associated with a significant decrease in progression through embryo stages during the seven days of in vitro development and with an increase in morphologically abnormal embryos. Progression through embryo stages during four days of in vivo development was also inhibited by ethanol pretreatment of dams At 99 h post-hCG of in vivo development, there were fewer total,hatched, and expanded blastocysts, and a complete absence of implanting blastocysts among females treated with ethanol. In summary, low-dose chronic ethanol consumption of sexually maturing female mice prior to conception has adverse effects on preimplantation embryodevelopment, both under in vitro and in vivo conditions, manifested as retarded development, embryo anormalities, and a reduction in expansion and hatching of the preimplantation blastocyst.