INVESTIGADORES
CEBRAL Elisa
artículos
Título:
19- Alterations in preimplantation in-vivo development after preconceptional chronic moderate alcohol consumption, in female mice.
Autor/es:
CEBRAL E; LASSERRE A; RETTORI V; GIMENO MAF
Revista:
ALCOHOL AND ALCOHOLISM
Editorial:
OXFORD UNIV PRESS
Referencias:
Lugar: Oxford; Año: 2000 vol. 35 p. 336 - 343
ISSN:
0735-0414
Resumen:
The susceptibility of preimplantation stages of embryo development after preconceptional alcohol ingestion by females has been little explored. Recently we have shown that chronic 10 % w/v ethanol intake by young female mice reduces the ovulatory response and impaires the quality of the oocytes. The aim of this study was to investigate the effects of 10% ethanol administration for 30 days to immature female mice on the day of in vitro fertilization (day 1) and preimplantation embryo development. Female mice were ovulated on day 27 and 29 of the ethanol treatment, and the in vitro fertilization was performed at 16 h post-HCG (day 30). The oocytes from the ethanol-treated females inseminated with spermatozoa from control males, showed a significant high percentage of parthenogenetic activation (II PB and 1PN) as compared to the control females. Also, increased percentage of fragmented oocytes was found after insemination as compared to that of control females (p<0.001). When the embryos were cultured, the percentage of 2-cell (day 2), 4-cell (day 3) embryos and compacted morulae (day 4) was significantly reduced in the treated females as compared to control females. At day 5, we found very significantly decreased percentage of early and expanded blastocysts in the ethanol-treated females. The percentage of hatching and hatched (extruded) blastocysts was also significantly reduced in the treated females at days 6 and 7 (blastocyst stages). More increased percentage of morphologically abnormal embryos were found on day 5 and 6 in the ethanol-treated females than in the controls. In summary, the chronic moderate ethanol ingestion by the young female mice resulted in decreased fertilization, embryo growth retardation, cleavage arrest and abnormal embryos in vitro development.
