INVESTIGADORES
PERONE Marcelo Javier
artículos
Título:
Curcumin acts anti-proliferative and pro-apoptotic in human meningiomas
Autor/es:
CURIC, S; WU, Y; SHAN, B; SCHAAF, C; UTPADEL, D; LANGE, M; KUHLEN, D; PERONE, MJ; ARZT, E; STALLA, GK; RENNER, U
Revista:
JOURNAL OF NEURO-ONCOLOGY.
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2013 vol. 113 p. 385 - 396
ISSN:
0167-594X
Resumen:
Meningiomas, the most frequent benign intracranial
and intraspinal types of tumors are normally
removed by surgery. Complications can occur when the
tumor is critically localized and cannot be completely
removed or when comorbidities of the mostly elder patients
increase the general surgical risk. Thus, alternate medical
treatment concepts for the therapy of meningiomas would
be desirable. Curcumin, the active ingredient of the spice
plant Curcuma longa has shown anti-tumorigenic actions
in many different types of tumors and therefore, its effect
on growth and apoptosis of meningioma cells was studied
in the present paper. In vitro, treatment of the human Ben-
Men-1 meningioma cell line and of a series of 21 primary
human meningioma cell cultures with curcumin (120 lM)
strongly reduced the proliferation in all cases in a dose
dependent manner. Cell cycle analysis by fluorescenceactivated
cell sorting showed growth arrest at G2/M phase,
which was confirmed by demonstrating the corresponding
modulation of proteins involved in G2/M arrest by
immunoblotting and/or confocal laser microscopy. High
dosages (20, 50 lM) of curcumin induced a significant
increase of apoptosis in Ben-Men-1 and primary meningioma
cell cultures as demonstrated by morphological
changes of cell nuclei, DNA fragmentation, translocation
of cell membrane associated phosphatidyl serine and the
induction of apoptotic-acting cleaved caspase-3. Our
results suggest that the multi-targeting drug curcumin has
potent anti-tumorigenic actions in meningioma cells and
might therefore be a putative candidate for the pharmacological
treatment of meningiomas.