CERZOS   05458
CENTRO DE RECURSOS NATURALES RENOVABLES DE LA ZONA SEMIARIDA
Unidad Ejecutora - UE
artículos
Título:
Isolation and characterization of atrazine-degrading Arthrobacter sp. strains from Argentine agricultural soils
Autor/es:
FERNÁNDEZ, L.; VALVERDE C; GÓMEZ, MARISA A
Revista:
ANNALS OF MICROBIOLOGY
Editorial:
INST MICROBIOLOGIA
Referencias:
Lugar: Milan; Año: 2012
ISSN:
1590-4261
Resumen:
Three bacterial strains capable of degrading atrazine
were isolated from Manfredi soils (Argentine) using
enrichment culture techniques. These soils were used to
grow corn and were treated with atrazine for weed control
during 3 years. The strains were nonmotile Gram-positive
bacilli which formed cleared zones on atrazine solid medium,
and the 16S rDNA sequences indicated that they were
Arthrobacter sp. strains. The atrazine-degrading activity of
the isolates was characterized by the ability to grow with
atrazine as the sole nitrogen source, the concomitant herbicide
disappearance, and the chloride release. The atrazinedegrader
strain Pseudomonas sp. ADP was used for comparative
purposes. According to the results, all of the isolates
used atrazine as sole source of nitrogen, and sucrose
and sodium citrate as the carbon sources for growth. HPLC
analyses confirmed herbicide clearance. PCR analysis
revealed the presence of the atrazine catabolic genes trzN,
atzB, and atzC. The results of this work lead to a better
understanding of microbial degradation activity in order to
consider the potential application of the isolated strains in
bioremediation of atrazine-polluted agricultural soils in
Argentina.