Arginine metabolic pathways involved in the modulation of tumor-induced angiogenesis by macrophages

LILIA ELENA DAVEL; MARIA ADELA JASNIS; EULALIA DE LA TORRE; TOMOMI GOTOH; MIRIAM DIAMENT; GABRIELA MAGENTA; EUGENIS SACERDOTE DE LUSTIG ; MARÍA ELENA SALES

FEBS LETTERS

Elsevier Science B.V.

Lugar: Netherlands; Año: 2002 vol. 532 p. 216 - 220

0014-5793

Neovascularization, an essential step for tumor progression and metastasis development, can be modulated by the presence of macrophages (Mps) in the tumor microenvironment. The ability of Mps to regulate the angiogenicity of the LMM3 tumor cell line was studied. Peritoneal Mps from LMM3tumor- bearing mice (TMps) potentiate in vivo LMM3angiogenicity. These results were con¢rmed by CD31 immunoblotting assays. The activity of TMps depended on nitric oxide synthase (NOS) and arginase (A) activity. By immunoblotting we evidenced that AI and AII isoforms were up-regulated in TMps while the inducible and neuronal NOS isoforms were highly expressed in normal Mps. TMps might positively modulate tumor growth by stimulating angiogenic cascade mainly through polyamine synthesis.