INVESTIGADORES
GOMEZ CASATI Diego Fabian
artículos
Título:
Deficiency of Arabidopsis thaliana frataxin alters mitochondrial Fe-S proteins activity and induces oxidative stress
Autor/es:
BUSI, M. V.; MALIANDI, M. V.; VALDEZ, H.; CLEMENTE, M.; EDUARDO ZABALETA; ARAYA, A.; DIEGO FABIAN GOMEZ CASATI
Revista:
Plant Journal
Editorial:
Blackwell Publishing
Referencias:
Año: 2006 vol. 48 p. 873 - 882
ISSN:
0960-7412
Resumen:
Frataxin, a protein crucial for the biogenesis of mitochondria in different organisms, was recently identified in
Arabidopsis thaliana. To investigate the role of frataxin in higher plants, we analyze two knock-out and one
knock-down T-DNA insertion mutants. The knock-out mutants present an embryo-lethal phenotype, indicating
an essential role for frataxin. The knock-down mutant has reduced frataxin mRNA and protein levels. This
mutant also presents retarded growth, reduced fresh weight of fruits and reduced number of seeds per fruit.
Surprisingly, transcription of aconitase and the FeS subunit of succinate dehydrogenase (SDH2-1) are
increased in mutant plants; however, the activity of these proteins is reduced, indicating a role for frataxin in
FeS cluster assembly or insertion of FeS clusters into proteins. Mutant plants also have increased CO2. To investigate the role of frataxin in higher plants, we analyze two knock-out and one
knock-down T-DNA insertion mutants. The knock-out mutants present an embryo-lethal phenotype, indicating
an essential role for frataxin. The knock-down mutant has reduced frataxin mRNA and protein levels. This
mutant also presents retarded growth, reduced fresh weight of fruits and reduced number of seeds per fruit.
Surprisingly, transcription of aconitase and the FeS subunit of succinate dehydrogenase (SDH2-1) are
increased in mutant plants; however, the activity of these proteins is reduced, indicating a role for frataxin in
FeS cluster assembly or insertion of FeS clusters into proteins. Mutant plants also have increased CO2SDH2-1) are
increased in mutant plants; however, the activity of these proteins is reduced, indicating a role for frataxin in
FeS cluster assembly or insertion of FeS clusters into proteins. Mutant plants also have increased CO22
assimilation rates, exhibit increased formation of reactive oxygen species (ROS) and have increased levels of
transcripts for proteins known to be involved in the ROS stress responses. These results indicate that frataxin
is an essential protein in plants, required for full activity of mitochondrial FeS proteins and playing a
protective role against oxidative damage.