INVESTIGADORES
LORES ARNAIZ Silvia
artículos
Título:
Reactions of peroxynitrite in the mitochondrial matrix.
Autor/es:
VALDEZ, L.; ALVAREZ, S.; LORES ARNAIZ, S.; SCHOPFER, F.; PODEROSO, J.J. ; BOVERIS, A.
Revista:
FREE RADICAL BIOLOGY AND MEDICINE
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Lugar: Amsterdam; Año: 2000 vol. 29 p. 349 - 356
ISSN:
0891-5849
Resumen:
Superoxide radical (O22
2) and nitric oxide (NO) produced at the mitochondrial inner membrane react to form
peroxynitrite (ONOO2) in the mitochondrial matrix. Intramitochondrial ONOO2 effectively reacts with a few biomolecules
according to reaction constants and intramitochondrial concentrations. The second-order reaction constants (in
M21 s21) of ONOO2 with NADH (233 6 27), ubiquinol-0 (485 6 54) and GSH (183 6 12) were determined
fluorometrically by a simple competition assay of product formation. The oxidation of the components of the
mitochondrial matrix by ONOO2 was also followed in the presence of CO2, to assess the reactivity of the nitrosoperoxocarboxylate
adduct (ONOOCO2) and nitric oxide (NO) produced at the mitochondrial inner membrane react to form
peroxynitrite (ONOO2) in the mitochondrial matrix. Intramitochondrial ONOO2 effectively reacts with a few biomolecules
according to reaction constants and intramitochondrial concentrations. The second-order reaction constants (in
M21 s21) of ONOO2 with NADH (233 6 27), ubiquinol-0 (485 6 54) and GSH (183 6 12) were determined
fluorometrically by a simple competition assay of product formation. The oxidation of the components of the
mitochondrial matrix by ONOO2 was also followed in the presence of CO2, to assess the reactivity of the nitrosoperoxocarboxylate
adduct (ONOOCO22) in the mitochondrial matrix. Intramitochondrial ONOO2 effectively reacts with a few biomolecules
according to reaction constants and intramitochondrial concentrations. The second-order reaction constants (in
M21 s21) of ONOO2 with NADH (233 6 27), ubiquinol-0 (485 6 54) and GSH (183 6 12) were determined
fluorometrically by a simple competition assay of product formation. The oxidation of the components of the
mitochondrial matrix by ONOO2 was also followed in the presence of CO2, to assess the reactivity of the nitrosoperoxocarboxylate
adduct (ONOOCO221 s21) of ONOO2 with NADH (233 6 27), ubiquinol-0 (485 6 54) and GSH (183 6 12) were determined
fluorometrically by a simple competition assay of product formation. The oxidation of the components of the
mitochondrial matrix by ONOO2 was also followed in the presence of CO2, to assess the reactivity of the nitrosoperoxocarboxylate
adduct (ONOOCO22 was also followed in the presence of CO2, to assess the reactivity of the nitrosoperoxocarboxylate
adduct (ONOOCO22
2) towards the same reductants. The ratio of product formation was about similar
both in the presence of 2.5 mM CO2 and in air-equilibrated conditions. Liver submitochondrial particles supplemented
with 0.252 mM ONOO2 showed a O2) towards the same reductants. The ratio of product formation was about similar
both in the presence of 2.5 mM CO2 and in air-equilibrated conditions. Liver submitochondrial particles supplemented
with 0.252 mM ONOO2 showed a O22 and in air-equilibrated conditions. Liver submitochondrial particles supplemented
with 0.252 mM ONOO2 showed a O2mM ONOO2 showed a O2
2 production that indicated ubisemiquinone formation and autooxidation. The
nitration of mitochondrial proteins produced after addition of 200 mM ONOO2 was observed by Western blot analysis.
Protein nitration was prevented by the addition of 50200 mM ubiquinol-0 or GSH. An intramitochondrial steady state
concentration of abouproduction that indicated ubisemiquinone formation and autooxidation. The
nitration of mitochondrial proteins produced after addition of 200 mM ONOO2 was observed by Western blot analysis.
Protein nitration was prevented by the addition of 50200 mM ubiquinol-0 or GSH. An intramitochondrial steady state
concentration of aboumM ONOO2 was observed by Western blot analysis.
Protein nitration was prevented by the addition of 50200 mM ubiquinol-0 or GSH. An intramitochondrial steady state
concentration of aboumM ubiquinol-0 or GSH. An intramitochondrial steady state
concentration of abou