INVESTIGADORES
BIANCO Ismael Dario
artículos
Título:
Signals elicited at the intestinal epithelium upon chitosan feeding contribute to immunomodulatory activity and biocompatibility of the polysaccharide.
Autor/es:
CANALI M.M.; PORPORATTO C; AOKI, M.P.; BIANCO I.D.; CORREA S.G.
Revista:
VACCINE
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2010 vol. 28 p. 5718 - 5724
ISSN:
0264-410X
Resumen:
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Chitosan is a copolymer of
N-acetylglucosamine and glucosamine derived from chitin with several
applications in pharmaceutical and medical fields. This
polysaccharide exhibits adjuvant properties in mucosal
immune
responses of humans, rats and mice. Characterization of signals
elicited by chitosan at the intestinal epithelium could explain its
immunomodulatory activity and biocompatibility. We fed normal rats
with single doses of chitosan and 16 h later, we purified intestinal
epithelial cells (IECs) to assess immune and biochemical parameters.
Following chitosan administration, mRNA expression and release of
several cytokines and chemokines increased, injury markers maintained
constitutive levels and MHC type II molecule expression was
augmented. IEC supernatants showed higher levels of IL-10, IL-6 and
TGF-β. Arginase activity
of IECs increased upon chitosan interaction in vivo and in vitro.
Together, after chitosan feeding, mild activation of IECs occurs in
vivo, with production of regulatory factors that could be relevant
for its biocompatibility and immunomodulatory effects.