Candida albicans Tpk1p and Tpk2p isoforms differentially regulate pseudohyphal development, biofilm structure, cell aggregation and adhesins expression

ROMINA GIACOMETTI; KRONBERG FLORENCIA; BIONDI RICARDO MIGUEL; PASSERON SUSANA

YEAST

JOHN WILEY & SONS LTD

Año: 2011 vol. 28 p. 293 - 308

0749-503X

Candida albicans undergoes a reversible morphological transition from single yeast cells to pseudohyphal and hyphal filaments. In this organism PKA, coded by two catalytic subunits (TPK1 and TPK2) and one regulatory subunit (BCY1), mediates basic cellular processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different to pseudohyphal and hyphal filaments. In this organism PKA, coded by two catalytic subunits (TPK1 and TPK2) and one regulatory subunit (BCY1), mediates basic cellular processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different to pseudohyphal and hyphal filaments. In this organism PKA, coded by two catalytic subunits (TPK1 and TPK2) and one regulatory subunit (BCY1), mediates basic cellular processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different to pseudohyphal and hyphal filaments. In this organism PKA, coded by two catalytic subunits (TPK1 and TPK2) and one regulatory subunit (BCY1), mediates basic cellular processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different to pseudohyphal and hyphal filaments. In this organism PKA, coded by two catalytic subunits (TPK1 and TPK2) and one regulatory subunit (BCY1), mediates basic cellular processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different processes, such as the yeast to hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, though distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different