INVESTIGADORES
ALVAREZ Silvia
artículos
Título:
Lung oxidative metabolism after exposure to ambient particles
Autor/es:
MAGNANI, NATALIA; MARCHINI, TIMOTEO; TASAT, DEBORAH; ALVAREZ, SILVIA; EVELSON, PABLO
Revista:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Año: 2011 vol. 412 p. 667 - 672
ISSN:
0006-291X
Resumen:
The aim of this work was to study the time course of the oxidative metabolism in mice lung after exposure
to ambient particles (ROFA). Swiss mice were intranasally instilled with a ROFA suspension
(0.20 mg/kg). Animals were sacrificed 1 or 3 h after the exposure. Eighty percentage of increased oxygen
consumption was observed in tissue cubes after 1 h of exposure. This observation was accompanied by an
increased NADPH oxidase activity (40%) and mitochondrial oxygen consumption in state 3 (19%). NO production
by lung homogenates was found to be increased by 43% after 3 h of exposure. Phospholipid oxidation
in lung homogenates showed a 29% increase after 1 h of exposure, while a 30% increase in the
carbonyl content was found only after 3 h of exposure. Our data show the relative importance of different
sources of reactive oxygen species (NADPH oxidase activity and mitochondrial respiration) to the
increased tissue oxygen consumption, oxidative damage and antioxidant status observed in an acute
model of ROFA particles exposure.