Cloning of IP15, a pancreatitis induced gene whose expression inhibits cell growth.

ALEJANDRO JAVIER ROPOLO; TOMASINI, R.; GRASSO, D.; DUSETTI, N. J.; CERQUETTI, M. C.; IOVANNA, J. L.; VACCARO, M. I.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ELSEVIER

Lugar: Amsterdam, The Netherlands.; Año: 2004 vol. 319 p. 1000 - 1009

0006-291X

We describe the cloning and expression of the mouse gene interferon-inducible-protein 15 (IP15), whose activation is related to the acute phase of experimental pancreatitis. Analysis of its structure indicates that it encodes a putative transmembrane protein of 137 amino acids. This gene contains a predicted IFN-stimulable-response element. In vivo studies showed that IP15 is strongly activated in pancreas early during caerulein-induced pancreatitis. In situ hybridization of IP15 mRNA showed that its expression is restricted to acinar cells. IP15 was also induced in pancreas under systemic-lipopolysaccharide treatment and in intestine under Salmonella infection. In vitro studies using NIH3T3 fibroblasts showed that IP15 is induced by IFN-alpha. Growth rate was significantly lower in cells transfected with pcDNA4/IP15 plasmid. In addition, cells expressing IP15 showed less capacity to develop colonies after antibiotic selection. In conclusion, we identified a new interferon-inducible gene that is activated early in pancreas with pancreatitis and whose expression inhibits cell growth.