Quantitation of three benzimidazole compounds in milk combining effervescence-assisted dispersive liquid–liquid microextraction with electrochemical detection using carbon nanotube-electropolymerized L-DOPA modified sensor

TEGLIA, CARLA MARÍA; GEGENSCHATZ, SOFIA MARÍA ; MONZON, CELINA MARÍA; GOICOECHEA, HECTOR CASIMIRO; GUTIERREZ, FABIANA A

MICROCHEMICAL JOURNAL

ELSEVIER SCIENCE BV

Año: 2024 vol. 201

0026-265X

enzimidazoles constitute a group of compounds known for their applications in pharmacy, agriculture and foodindustry. In recent decades, researchers have dedicated significant efforts to improving microextraction anddetection methods that align with the principles of green analytical chemistry. In the present study, we havedevised an effervescence-assisted dispersive liquid–liquid microextraction method and built an electrochemicalsensor for the detection of albendazole, fenbendazole, and thiabendazole using GCEs (glassy carbon electrode)modified with multiple walled carbon nanotubes (MWCNTs) and poly (L-Dopa) film, allowing benzimidazoledetection in complex matrices such as milk. Optimization of the microextraction procedure and electrochemicalperformance of the sensor was done by response surface methodology. The electrochemical sensor enabled thedetection of albendazole, fenbendazole, and thiabendazole in a range of 0.37 to 100 μg mL􀀀 1 with a detectionlimit ranged between 0.14 to 0.28 μg mL􀀀 1. The developed sensor was tested in commercial milk. Results showsatisfactory analytical performance, achieving a sensitive and selective quantification, especially considering thecomplexity of the matrix in which it was applied. The newly created sensor facilitates the simultaneous quantitationof the desired analytes, delivering an exceptionally praiseworthy analytical performance with detectionlimits lower than most existing methods.