INVESTIGADORES
GUBERMAN Alejandra Sonia
artículos
Título:
STAT5 transcriptional activity is impaired by LIF in a mammary epithelial cell line.
Autor/es:
AGUSTINA RODRIGUEZ GRANILLO, JUAN CARLOS BOFFI, LINO BARAÑAO, EDITH KORDON, ADALI PECCI Y ALEJANDRA GUBERMAN
Revista:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Referencias:
Año: 2007 vol. 356 p. 727 - 732
ISSN:
0006-291X
Resumen:
Gene regulation mediated by STAT factors has been implicated in cellular
functions with relevance to a variety of processes. Particularly, STAT5 and
STAT3 play a crucial role in mammary epithelium displaying reciprocal
activation kinetics during pregnancy, lactation and involution. Here, we show
that LIF treatment of mammary epithelial HC11 cells reduces the phosphorylation
levels and transcriptional activity of p-STAT5 in correlation with STAT3
phosphorylation. We have also
found that STAT5 activity is
negatively modulated by this cytokine, both on a gene whose expression is induced, as well as on a promoter
repressed by STAT5. Besides, our results show that lactogenic hormones
increase LIF effect on gene induction without modifying STAT3 phosphorylation state. Our findings
strongly suggest that there is crosstalk between STAT5 and STAT3 pathways that
could modulate their ability to regulate gene expression.