Effects of MCH/NEI in the striatum and interactions between them and with alpha-MSH on IP3 levels

MÓNICA SILVINA SANCHEZ, VICTORIA BERBERIAN, MAR´ıA ESTER CELIS

PEPTIDES

ELSEVIER SCIENCE INC

Año: 2002 vol. 23 p. 877 - 880

0196-9781

In this study, we investigated: (a) the effect of melanocyte concentrating hormone (MCH) and neuropeptide glutamine (E)-isoleusine-(I) (NEI) on IP3 production on an “in vitro” model using slices containing caudate putamen (CP) and accumbens nuclei (ACC); and (b) the interaction between these peptides and with α-melanocyte-stimulating-hormone (α-MSH) on the production of this second messenger. Only MCH at the highest dose studied (3.6 μM) increased the production of IP3, whereas at the low concentration (0.6 μM) it did not affect IP3 levels. NEI and α-MSH at both concentrations tested (0.6 and 3.6 μM), did not affect IP3 production either. However, when NEI or α-MSH (at 3.6 μM) were added together with 3.6 μM MCH, the increase in the IP3 content induced by this last peptide was blocked.