Fusarium sudanense, endophytic fungus causing typical symptoms of seedling blight and seed rot on wheat

LARRÁN, S.; SANTAMARINA SIURANA, M. PILAR; ROSELLÓ CASELLES, JOSEFA; SIMÓN MR; PERELLÓ, A

Journal of King Saud University ? Science

Springer

Año: 2020 vol. 32 p. 468 - 474

1018-3647

An endophytic fungus isolated from healthy wheat seeds cultivar Klein Yarará in Buenos Aires Province,Argentina, was initially identified as belonging to the Fusarium fujikuroi species complex based on morphological and cultural characteristics. The fungus role in pathogenicity was investigated by artificialinoculation of wheat seeds. Symptoms evaluated 7 and 14 days after inoculation showed that the funguswas pathogenic on seed and seedlings causing symptoms as seed decay, seedling blight and seed rot. Thefungus was re-isolated to fulfill Koch?s Postulates and was identified as F. sudanense (strain LBEA 3100), anew species recorded in Argentina. The fungal identity was corroborated using molecular techniques bysequencing the ITS region, D1/D2 domains of the LSU gene and TEF-1 alpha region and by comparisonwith international databases. Ecophysiological studies of F. sudanense (LBEA 3100) performed at differentwater activities and temperatures showed faster growth rate at the highest water activity and 25 C. Thisis the first report of F. sudanense, isolated from healthy wheat seeds, causing typical symptoms of seedlingblight and seed rot on wheat.