Effect on the growth and development and induction of abnormalities by a glyphosate commercial formulation and its active ingredient during two developmental stages of the South-American Creole frog, Leptodactylus latrans

BACH N.; NATALE G.S.; SOMOZA M.G.; RONCO A.E.

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2016 vol. 23 p. 23959 - 23971

0944-1344

We evaluated the acute lethal and sublethal effectsof technical-grade glyphosate (GLY) and the GLY-based commercialformulation Roundup ULTRA MAX® (RU) on twoGosner stages (Gss) 25 and 36 of the South-American Creolefrog, Leptodactylus latrans. Bioassays were performedfollowing standardized methods within a wide range of concentrations(0.0007?9.62 mg of acid equivalents per liter?a.e./L?of RU and 3?300 mg/L of GLY). The endpoints evaluatedwere mortality, swimming activity, growth, development,and the presence of morphologic abnormalities, especiallyin the mouthparts. No lethal effects were observed onlarvae exposed to GLY during either Gs-25 or Gs-36. Theconcentrations inducing 50 % lethality in RU-exposed larvaeat different exposure times and Gss ranged from 3.26 to9.61 mg a.e./L. Swimming activity was affected by onlyRU. Effects on growth and development and the inductionof morphologic abnormalities?like oral abnormalities andedema?were observed after exposure to either GLY or RU.Gs-25 was the most sensitive stage to both forms of the herbicide.The commercial formulation was much more toxicthan the active ingredient on all the endpoints assessed.Effects on growth, development, and the induction of morphologicabnormalities observed in the range of environmentalconcentrations reported for agroecosystems of Argentina constitutean alert to the potential detrimental effects of the herbicidethat could be affecting the fitness and survival of anuransin agroecosystems.