Molecular detection and phylogenetic analysis of Anaplasma platys-like and Candidatus Anaplasma boleense strains from Argentina

SEBASTIAN PS, MAZZUCCO PANIZZA MN, GRACÌA RÌOS IJM, TARRAGONA EL, TROVA GB, SÀNCHEZ NEGRETTE O, PRIMO ME, NAVA S

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2023

0147-9571

The present study aimed at the molecular detection of Anaplasma spp. in different samples obtained from cattle,goats and free-living Rhipicephalus microplus ticks from Argentina. DNA of members of the Anaplasmataceaefamily was detected by different PCR assays. The phylogenetic analyses of the obtained partial DNA sequences ofthe 16 S rDNA gene resulted in the identification of two different Anaplasma spp.: (I) Anaplasma platys-likebacteria (in blood sample from cattle and pools of R. microplus larvae and (II) Candidatus Anaplasma boleense (inblood samples from goats and one pool of R. microplus larvae of R. microplus). Candidatus A. boleense was foundin two provinces that belong to different biogeographic regions, which leads to the conclusion that this bacteriummay be widely distributed in Argentina. Interestingly, both Anaplasma spp. were found in the same R. micropluspopulation in Chaco province, indicating that these two strains of Anaplasma are circulating in the same tickpopulation. The results of this work represent the first report of the circulation of A. platys-like bacteria and Ca.A. boleense in domestic ruminants and free-living R. microplus ticks in Argentina. Further studies to determinethe prevalence of infection, dispersion, clinical impact, transmission routes and cross-reactivity in serologicaltests of both Anaplasma species are needed.