INVESTIGADORES
LUCANGIOLI Silvia Edith
artículos
Título:
Development and validation of a novel sensitive UV-direct capillary electrophoresis method for quantification of alendronate in release studies from biomaterials
Autor/es:
CATTALINI JUAN; MOURINO V.; LUCANGIOLI S.
Revista:
ELECTROPHORESIS
Editorial:
WILEY-V C H VERLAG GMBH
Referencias:
Lugar: Weinheim; Año: 2018 vol. 39 p. 616 - 619
ISSN:
0173-0835
Resumen:
A simple, highly sensitive, and robust CE method applied to the determination of alendronate(ALN) was developed from matrices for tissue engineering, characterized by beinghighly complex systems. The novel method was based on the ALN derivatization with ophthalaldehydeand 2-mercaptoethanol for direct ultraviolet detection at 254 nm. The BGEconsisted of 20 mM sodium borate buffer at pH 10, and the electrophoretic parameterswere optimized.The method was validated in terms of specificity, linearity, LOD, LOQ,precision, accuracy, and robustness. The LOD and LOQ obtained were 0.8 and 2.7 g/mL,respectively. In addition, the method offers higher sensitivity and specificity compared toother CE and HPLC methods using UV-detectors, as well as low cost and simplicity thatallowed the rapid and simple quantitation of ALN from bone regeneration matrices.